CoSyn: Efficient Single-Cell Analysis Using a Hybrid Microfluidic Platform

Mohamed Ibrahim1, Krishnendu Chakrabarty1 and Ulf Schlichtmann2
1Department of Electrical and Computer Engineering, Duke University, Durham, NC 27708, USA
2Institute for Electronic Design Automation, Technical University of Munich, 80333 Munich, Germany


Single-cell genomics is used to advance our understanding of diseases such as cancer. Microfluidic solutions have recently been developed to classify cell types or perform singlecell biochemical analysis on pre-isolated types of cells. However, new techniques are needed to efficiently classify cells and conduct biochemical experiments on multiple cell types concurrently. System integration and design automation are major challenges in this context. To overcome these challenges, we present a hybrid microfluidic platform that enables complete single-cell analysis on a heterogeneous pool of cells. We combine this architecture with an associated design-automation and optimization framework, referred to as Co-Synthesis (CoSyn). The proposed framework employs real-time resource allocation to coordinate the progression of concurrent cell analysis. Simulation results show that CoSyn efficiently utilizes platform resources and outperforms baseline techniques.

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